Florence is a city in Lyon County, Minnesota, United States. The population was 28 at the 2020 census. Florence was platted in 1888, …Florence is a city in Lyon County, Minnesota, United States. The population was 28 at the 2020 census. Florence was platted in 1888, and named for Florence Sherman, the daughter of a first settler. A post office was in operation at Florence in 1889.