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Normally, single-molecule FRET microscopes cost upwards of half-a-million dollars, but scientists and students at Sheffield were able to make the smfBox for roughly $50,000.
FRET X can identify where specific amino acids and post-translational modifications are located in a single full-length protein molecule.
Definition: Förster Resonance Energy Transfer (FRET) is a distance-dependent interaction between the electronic excited states of two dye molecules, where energy is transferred from a donor molecule ...
Researchers report single molecule studies of energy transfer using a quantum dot donor with an organic dye acceptor. They demonstrate that suppression of the blinking of the QD donor minimizes the ...
Researchers flip the switch at the nanoscale by applying light to induce bonding for single-molecule device switching.
This flow cell is then placed onto a single-molecule fluorescence microscope using a 3D-printed adapter, allowing researchers to observe the real-time dynamics of individual molecules across ...
Researchers have demonstrated a switch, analogous to a transistor, made from a single molecule called fullerene. By using a carefully tuned laser pulse, the researchers are able to use fullerene ...
Future directions This research addresses a critical hurdle in the advancement of nanoscale devices by offering a method to precisely control single-molecule reaction dynamics.
The team has been using FRET-FLIM to study the activity of proteins in dendritic spines, protrusions that form off of neuronal branches, and make synaptic connections and communicate with other ...
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