Figure 1: Schematic representation of the continuous FFE apparatus coupled off-line to RP-HPLC. Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; ...

Evaluation of: Stephanowitz H, Lange S, Lang D et al. Improved two-dimensional reversed phase–reversed phase LC-MS/MS approach for identification of peptide–protein interactions. J. Proteome Res. 11(2 ...

Given the nature of biomolecules, such as amino acids and peptides, many HPLC reversed phase bio-separations often require ion-pairing reagents, such as Trifluoroacetic acid (TFA), in combination with ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

Supercritical fluid chromatography, or SFC, has been around for decades. During that time, the separation technique has fallen in and out of and back in favor. But it has consistently been relegated ...

Gradient elution involves a programmed change in mobile-phase composition over time, transitioning from weak to strong during a separation. Linear gradients, characterized by a constant change in ...

An interview with Dr. Michael Dong, about his upcoming talk at Pittcon. Why are stability-indicating methods important in pharmaceutical analysis? The stability-indicating assay is perhaps the single ...

When coupled with mass spectrometry, liquid chromatography forms a powerful technique with high sensitivity and selectivity. The technology is especially relevant to the specific detection and ...