Supporting evidence of biochemical assays and single-molecule FRET (smFRET) experiments confirmed that DNA unwrapping suppresses Chd1 activity not by physically blocking it, but through allosteric ...

By binding there, the molecule enhances the overall stability of the protein; this, in turn, boosts its ability to replicate and repair DNA, regardless of whether a mutation is present.

We present a technique that combines the power of single-molecule spectroscopy to separate subpopulations in a heterogeneous ensemble with submicrosecond correlation spectroscopy based on a Hanbury ...

To demonstrate precise distance measurement and accuracy, the research team used a molecule with which the FRET method actually started off in a classic experiment in the 1960s.

FRET X can identify where specific amino acids and post-translational modifications are located in a single full-length protein molecule.

For example, dye photobleaching is substantially suppressed, which is clearly visible in the lower panel of Figure 1 (B). This is an advantage for single molecule biological sensing and imaging.

Using single-molecule FRET spectroscopy and detailed structural information from coarse-grained simulations, we observe and characterize three interconvertible states representing an open and kissing ...

This review is focused on the advantages of the employment of single-molecule Forster’s resonance energy transfer (sm-FRET), which is worthwhile to access the dynamic architecture and structural ...

DNA oligonucleotides used in this study (Table S1); estimated interdye distances (R) as well as estimated and experimentally determined FRET efficiencies (E) (Table S2); the four-way sensor design ...